Friday, September 6, 2019
Reasons I Choose Belmont University Essay Example for Free
Reasons I Choose Belmont University Essay One of the universities that have caught my attention was the Belmont University. Coming from a Christian Community, I felt that the university can help me develop into a more mature Christian. The schools mission is to provide an to its education that is academically challenging, that promotes the empowerment of both men and women. Their diverse backgrounds help hone the students to become engaged with current issues, and in the improvement of the society, through their compassion, courage, discipline, and faith in God. Since the university is centered on the needs of its students, the university provided an environment that helped its students grow spiritually and intellectually. With the help of their peers and professors, the students are able to help themselves become the best in their chosen field. In addition to this, the university offers a wide variety of courses that cater to each individuals interest and needs. Admission to the university definitely allows an individual to grow and mature to become a better person, both intellectually and spiritually. Other factors are also taken into great consideration, such as their social and physical needs. The diversity and artistic favor that this university has can help in the building of strong foundations for the future of its students. Belmont does not only offer a conducive environment suitable for learning, but also caters to an environment contributory to spiritual growth. In addition to this, the universitys location is beyond par. Since Nashville is known to be a ââ¬Å"Music Cityâ⬠, the university can help hone its students to become excellent in their field by providing world class performances. After acquiring a degree form Belmont, anyone can easily find jobs just outside of the university, with the wide array of job offerings in Nashville. Church-related activities may still be practiced, through the number of churches near the vicinity. The Belmont University is definitely the answer to all our prayers.
Thursday, September 5, 2019
Analysis of Bacteria Strains: Phonotypical Tests
Analysis of Bacteria Strains: Phonotypical Tests Kunthavai Jeevananthan Bacterial Identification Aims: To analyse and identify 10 different strains of bacteria by conducting 19 different tests phonotypical tests. Bacteria also known as eubacteria are microorganisms that are invisible to the naked eye but exist in virtually all environments in the world. Bacteria are classified as part of the Monera kingdom which includes archaebacteria and cyanobacteria. Most bacteria are pathogenic or disease causing however not all bacteria are harmful as there are a number of bacteria that can be found in the human body that have positive benefit to their hosts such as help digest food, secrete hormones, chemicals and vitamins required in cell metabolism and even fight off other harmful bacteria. Bacteria exist in various rod, spiral and spherical shapes and are more numerous than any other living organisms. It is important to be able to identify microorganisms in medical clinic in order to help selection of antibodies. Some pharmaceutical products are also made using bacteria therefore many unknown and unidentified bacteria may be useful in the clinical industries. The taxonomy or a particular bacterial c haracteristic can be used to identify similarities that show relationships with disease related descriptions (Janda and Abbott, 2002). Various laboratory tests have been developed that are based on the type of nutrients a bacterium can grow on, the kind of toxins or waste products they produce or how much variation in growth temperature they can tolerate and their morphology can be used to distinguish closely related strains of bacteria. Rapid test kits have also been developed to identify bacteria in the Enterobacteriaceae genera and other gram negative bacteria. Phonotypical approaches of identifying bacteria does not always provide sufficient information to set taxonomic boundaries between different species the repetition of some phenotypic characteristics make it difficult to separate them. Genotyping is however is more precise when it comes to differentiating bacteria within species that lead to the development of DNA hybridisation. This is a technique used measure the similarities in sequences between the DNA of an isolate and a known bacteria. Before conducting a phonotypical bacterial identification it is important to have a pure culture of the bacteria that needs to be identified so that all components of the cells have grown from a single cell and they are clones of one another also known as Holy Grail (Barrow and Feltham, 1993). The bacterial colonies formed for different types of bacteria have different cultural characteristics on agar plates known as colony morphology these include pigments, size edge, pattern, opacity and shine t herefore macroscopically examining the colonies of bacterial cultures is one of the first important tests in bacterial identification. There may be drawbacks in this technique as the visual interpretation can differ from person to person therefore it might not produce reliable results and also mutations in the bacteria strains occur all the time that may provide slightly different characteristics than normal making difficult or incorrect identification. A number of staining methods can be used to examine the cultures under a microscope such as negative staining which stains the background and leaves the cells clear so that the shape of the bacterial cells, presence of glistening capsule and presence of a diffusive extracellular substance (EPS) around the cell and the arrangement of cells can be determined. Differential staining is a test that divides bacteria into two large groups either gram negative or gram positive. Pink- red staining indicates gram negative bacteria and a blue purple staining shows gram positive bacteria cells. Unevenly stained clear surfaces can be formed on the surface of the bacterial cells during gram staining due to the presence of endospores that can be confirmed using spore stain. Acid fast staining is also used if cells appear long, slender and intertwined in order to confirm the presence of acid fast cells which are bacteria in the genera Mycobacteria and Nocardia that are resistant to gram staining. Oxidase, catalase and the ability of the culture to grow in anaerobic conditions are three tests that are conducted during the first steps of identification. Catalase test is to dip an inoculating needle coated with culture into a droplet of hydrogen peroxide and if the bacteria possesses a catalase enzyme it will breakdown the hydrogen peroxide into water and oxygen that effervesce to form foam. Catalase positive bacteria are usually aerobic while catalase negative bacteria are anaerobic. Cytochrome oxidase is another enzyme found normally in the electron transport chains of an aerobic bacteria and this is tested by adding an artificial substrate such as para amino dimethylaniline that will produce a dark red to black product when oxidised (Cullimore, 2000). Alongside these test the cultures can be tested on their abilities to grow under anaerobic conditions which can further divide them into 4 major groups; strictly aerobic, reduced concentrations of oxygen, both aerobic and anaero bic and strictly anaerobic bacteria. Urease synthesis, gelatin hydrolysis and citrate utilisation are tests that can be conducted to narrowly distinguish bacteria further to help identify their genera. Urease is produced the bacterial genera proteus, providentia and morgenella to break down urea into carbon dioxide and ammonia therefore it is a useful test to help distinguish these genera from other gram negative rods during identification. Urease test is carried out by incubating urea broth with samples of gram negative cultures. The presence of ammonia increases the pH hence turning the phenol red indicator to a pink-purple colour for a positive test (Harvey and Champe et al, 2001). The gelatin hydrolysis test identifies the ability of bacteria to produce gelatinases which can help identification of serratia and proteus. The citrate utilisation tests the ability of the bacteria to utilise citrate as its carbon and energy source used mostly to identify gram negative bacteria. Indole, methyl red- Voges- protease test and fermentation of glucose, sucrose and lactose are also test that are conducted to help assist with identification of bacteria. Indole test is preformed to test the ability of bacteria to breakdown amino acid tryptophan and produce indole that can be detected using Kovacââ¬â¢s reagent. This method is important in the identification of gram negative enterobacteria. Methyl red-Voges- Proskauer on the other hand are two tests that are conducted together as they both require the use of the same medium. The methyl red test identifies the ability of the bacteria to carry out mixed acid fermentations whereas the VP test determines whether the bacteria fermenting sugars via the butanediol pathway by testing for the by-product acetoin. These tests are also useful in differentiating between members of the enterobacteria such as E. coli (Wong, 2005). The ability of bacteria to ferment carbohydrates is also a way to discriminate them during identific ation as fewer bacteria are able to use disaccharides like lactose and sucrose as a source of energy. This can be detected by checking for release of gaseous by products and metabolic chemicals that are released during the process of oxidation and fermentation of sugars. These tests described in the context above were carried out under standard conditions and results were recorded. Results Table 1: shows test results for Colony Morphology for 10 unknown bacterial cultures A to J. Table 2: shows test results for 18 different bacterial identification tests for unknown cultures A to J. Urease, indole, citrate, oxidase, methyl red and Voges-Proskauer test were only carried out for gram negative strains of bacteria and the endospores were only tested for gram positive bacteria. Microphotographs showing cell morphologies and gram (+/-) strains for cultures C, D and F are shown in the appendix. For culture C it can be seen that the cells are arranged in ââ¬Å"grape likeâ⬠structures whereas C is arranged in packets of four. It can also be seen that culture F it can be seen that the cells were single and in chains. Discussion Organism A and B are both gram positive rods that gave positive results for anaerobic, catalase and endospores test however they can both be differentiated as organism B is brown in colour and a glucose fermenter whereas organism A is orange in colour and a non-glucose fermenter. Organism B was in a cooked meat liquid broth which also indicates that the bacterium maybe part of the Clostridium species that have a few pathogenic bacteria that are responsible for food poisoning and tetanus. Organism A is therefore Bacillus Cereus some bacterium in this species are harmless whereas others are pathogenic that may cause foodborne illnesses such as nausea, vomiting and diarrhoea. Organism C has the morphology of pale yellow cocci clusters arranged in packets of four. It is a gram positive bacterium that also shows positive results for glucose fermentation and the catalase test show characteristics of Staphylococcus species which include pathogenic bacteria that causes skin infections, pneum onia and food poisoning. Organism C appeared in clusters that were ââ¬Å"grape likeâ⬠and is also a gram positive cocci and has a positive result for catalase however it does not ferment carbohydrates but shows positive test results for their metabolic chemical produced and hereby conveying characteristics of Micrococcus species these bacteria are very rarely disease causing and if so some may cause chronic cutaneous infections (Breed and Murray, 1957). Organism E has a white flat mycelial morphology and it is a gram positive glucose fermenter therefore it is a part of the Streptomyces genera that are known to inhibit the soil and causes the common scab in root vegetables. Organism F is a clear gram negative rod bacterium that is glucose and lactose fermenter and produces metabolic chemicals for glucose, lactose. It also produced negative results for tests urease, gelatin, oxidase, Voges-Proskauer (VP) and indole however it showed positive results for catalase, citrate and methyl red and anaerobic tests as shown in figure 2. Analysing these results organism F can be identified as Escherichia coli that consist of many different strains some of which can cause urinary tract infections, diarrhoea, anaemia and even kidney failure. Organism G is a gram negative rod that produces metabolic chemicals for all three carbohydrates glucose, lactose and sucrose however bubbles were only present for lactose and sucrose. It also shows positive results for tests urease, indole anaerobic and VP but negative results for catalase, oxidase, gelatin and methyl red. Organism G showed similar results to organism F however unlike organism F it is urease positive therefore it can be a bacterium from the Proteus genera that also contain a few pathogenic bacteria that can cause urinary tract infections, kidney stones and cystitis. Organism H is only a glucose fermenter but does not produce any metabolic chemicals. It demonstrates positive test results for urease, gelatin, citrate, anaerobic catalase and oxidase but negative for both methyl red and VP as well as indole tests. It is possible that this bacterium maybe be a part of the Pseudomonas genera with different bacterial strains that may cause respiratory tract infections, dermatitis and bone and joint infections. Organism I is also a gram negative rod and had a clear pigmentation on an agar medium that is a glucose fermenter but however it produces metabolic chemicals for glucose, lactose and sucrose. It also produces positive results when being tested for citrate, anaerobic, catalase and methyl red but not for urease, gelatin, indole, oxidase and VP this indicates that organism I could be Salmonella typhimurium a pathogenic bacteria that causes gastroenteritis that leads to diarrhoea. Organism J is another gram negative bacterium that produces no bubbles for glucose, lactose or sucrose fermentation but produces metabolic chemicals for glucose and sucrose. It is also positive for gelatin, citrate, anaerobic, catalase and VP however it is negative for urease, indole, oxidase and methyl red tests. The red pigmentation of the bacterial culture and the other tests results indicates that organism J maybe Serratia marcescens that are associated with many different types of diseases some of which in clude bacteraemia, sepsis and meningitis. In order to conduct more specific identification of these bacteria further tests can be carried out that will help to distinguish each bacterium more accurately. Other tests that can be carried out include starch hydrolysis, lipid hydrolysis, motility (SIM) deeps, beta galactosidase, nitrate, coagulase, mannitol salt, osmotic pressure and haemolysis. References Barrow, G. I., Feltham, K. A. R. (1993). Crowan and Steelââ¬â¢s manual for the identification of medical bacteria: classification and nomenclature. 1-6. United Kingdom: Cambridge university press. Breed, R. S., Murray, G. D. E., Smith, N. R. (1957). Bergeyââ¬â¢s manual of determinative bacteriology. 695- 800. United States of America: Baltimore Williams and Wilkins co. Cullimore, D. R. (2000). Practical atlas for bacterial identification: initial stages of the identification of the bacterial culture. 1-7. United States of America: CRC press LLC; Harvey, R. A., Champe, P.C., Fisher, B.D. (2001). Microbiology: identification of bacteria. 24-27. United Stated of America: Lippincott Williams and Wilkins. Janda, J.M., Abbott, S. L. (2002). Bacterial identification for publication: when enough is enough? Journal of clinical microbiology. Vol. 40 no. 6, (1887-1891). Wong, T. (2005). Introduction to microbiology laboratory exercises for Allied Heath students: The IMViC tests. 48-50. United States of America: Author House. Appendix Culture C Culture D Culture F
Wednesday, September 4, 2019
The Wessex Tales Essay -- miscellaneous
The Wessex Tales Explore Hardyââ¬â¢s portrayal of women in three of the stories studied. The Wessex tales was set in the nineteenth century; Thomas Hardy decided to write his stories and novels in the past, during the nineteenth century before he was born. Hardy got some of his ideas from his grand parents; he used to spend long evening next to the fire listening to his grand parents telling stories form the past. Thomas Hardy invented his own places He is highlighting the point that women around that time do not have a say in any thing. The expectations of women were that they had a lower profile At this time people lived in small communities so every one knew each other, so gossip got around the community. If any one did any thing wrong then they became a victim of there own community, and would become isolated. Thomas Hardy portrays Rhoda s being a social victim for having a child out of wedlock, although she is isolated it is ironic that Farmer Lodge is not, but he is still accepted as part of the community. This shows women are lower than men women are inferior to men. Farmer lodge does not acknowledge the fact that he has a son ââ¬ËJust the same as usualââ¬â¢ ââ¬Ë Took no notice of youââ¬â¢ ââ¬ËNoneââ¬â¢ Rhoda son is use to his dad ignoring him. All that Farmer Lodge has given Rhoda is a job, Rhoda works away from the rest of the workers because she has been discarded. Hardy describes Rhoda as ââ¬ËFading women of thirtyââ¬â¢She looks older than she actually is; it also...
Tuesday, September 3, 2019
The Great Depression :: History
The Great Depression The Great Depression is comparable to Lennie and George's life. I would like to give a comparison of George Milton and Lennie Small to the Great Depression. The time that this story took place was during the Great Depression. John Steinbeck captured the reality of this most difficult time. During the Great Depression people needed to travel together to share chores and duties to make a living until something better came along. That is the way George and Lennie traveled. They traveled together to take care of each other but George took care of Lennie the most, because he was always getting in trouble. "You do bad things and I got to get you out." (Of Mice and Men p.11). During the Great Depression money was very scarce. You had to travel around to find a job in order to make money to survive. Lennie and George were in that type of predicament. Keeping enough money until the next job was difficult because prices were rising during the Great Depression and you had to budget your money. During this depression most people worked on farms because after the stock market crashed people realized that the reason the stock market crashed was because farms were not producing enough goods. People started to work on farms more to help everyone. Lennie and George worked for ranches and also in the fields. "He's a good skinner. He can rassel grain bags, drive a cultivator. He can do anything." (Of Mice and Men p.22). If you really look closely, George and Lennie's way of life and the Great Depression have a good deal in common. George and Lennie were outcasts in life. George Milton was small in size and a very smart man compared to Lennie. He was actually only of average intelligence. Lennie Small was a large person and very retarded. Since Lennie was so retarded he did not grasp the things that were happening around him. For example, if someone became upset about anything he would grab them and hold and squeeze until they stopped moving. Lennie would accidentally harm them and that is how he got inck smoke into the environment constantly. This disgusting smog stains buildings, covers trees, and hovers above many of our major city in large masses. Automobiles also contain some fluids that can be deadly to us and our wildlife. We often hear of gasoline spills by tanker trucks or by ships, both of which are meant to supply our automobiles with fuel.
Monday, September 2, 2019
Theme of Fate and Choice in Shakespeares Romeo and Juliet Essays
The Theme of Fate and Choice, in William Shakespeare's Romeo and Juliet In William Shakespeare's tragic play Romeo and Juliet, there are four quotes about fate and choice. The characters in the play choose their own fate, it happens because of their choices or actions. First, in the beginning of the play Romeo and Juliet are referred to as 'a pair of star-crossed lovers' (Romeo and Juliet, Prologue). Second, Romeo explains to Mercutio how he has a dream that if he goes to the Montague party he will die, but Romeo chooses to go to the party anyway. Third, Romeo kills Tybalt right after his marriage to Juliet. Fourth, Romeo decides to commit suicide because he hears of Juliet's death. The first quote on fate and choice is 'a pair of star crossed lovers' (Romeo and Juliet, Prologue). The above quote shows the theme of fate because the quote establishes to the audience that Romeo and Juliet are fated to die in the play. The second quote is I fear, too early, for my mind misgives Some consequence, yet hanging in the stars, Shall bitterly begin his fateful date With th...
Sunday, September 1, 2019
Egt Task
Although the current Managers are fluent in Korean, it will be important to hire local dialect persons to add to our staff. This will ensure acceptance, communications, and also preventing miss spoken words that me be taken as offensive If not done with the proper tone. Curriculum Instructor certification. Since South Korea converted the KIT- Korea Teakwood Escalation In 1954 Into the World Teakwood Federation, the feeling In Korea Is all Black Belts must hold certificates from the WTFO.This may be Just a financial ploy to collect money room those outside Korea, but the perception exists that if you hold a certificate (called a Okinawa Certificate) you are then legitimate regardless of what country you received the certificate, since all Okinawa certificates originate in Seoul, South Korea. All staff who do not currently hold their given Black Belt rank must be certified by the WTFO prior to submitting an application to be an instructor in our Korea Dugong expansion plans. Since no O kinawa officers exist in the united States, all certifications will be done by Head Master Ken Adulated, 6th Degree Black Belt Okinawa holder.Country of Origin of retail goods. Three primary countries manufacture martial arts apparel (uniforms) and sparring gear. These are Japan, China, and South Korea. While Americans are primarily obsessed by price, and seem to disregard country of orally pride, Korea Is the opposite. It will be critical that all uniforms, belts, and sparring gear ONLY come from South Korea manufacturing companies. To offer anything else is potentially offensive, but should these goods come from China or Japan, it would also be a disgrace since both countries attacked Korea in the last century. B. ProductThe product of Americas Best Teakwood is martial arts lessons. In America, most adults will not allow themselves to stick with a program, and many areas of the program we currently offer need to accommodate the lack of focus for Adult students, and their ability t o Justify quitting. Only 1 of 10 Americans (compared to 1 out of 4 Europeans) has attempted to learn martial arts. Of those 33 million, only 10% stay with It beyond 30 days, leaving 3. 3 million. Of those 3. 3 million only 10% will stay with It another 3-6 months quitting Jest as they prepare to test out of novice level.Of the enameling 330,000 who achieve Green belt/beginner Intermediate, only 10% of those will reach beginner advanced, or Brown Belt. We are left with just 33,000 Brown belts, In the USA, all but 10% quit after Just 30 days at Black belt, higher than any other country in the world. About 330 people are left who continue actual Black belt training, only 10% of those achieve Master level in the USA, Just 33 out of 330 million. Moving into Korea, we must modify our program for the long term goals of students.Parents will require their children attend, and they will attend with them, it will be a Emily event, training multiple days per week for 6-10 years or more. It wil l be important to modify our tuition timeline to not appear to be short sighted Americas with no ling term vision, or they may feel we are looking for short term profit, or will not stay through difficult times. The good news is that Korea has been losing the Gold Medals at the Olympics to other countries and is accepting of Americas teaching teakwood and they have fond respect for the Lopez family of Houston Texas, multiple Gold Medal winners.We will leverage our affiliation with the Amateur Athletic Union with Coach Lopez. We will use this for credibility but not for promoting an American Agency. 82. Price: Tuition pricing in America is structured around making it easy to quit, monthly pricing is not as important as not needing to make any commitment. In Korea it will be the opposite. Students will not mind signing non cancel agreements paid in full for 6 years as long as the rates are fair. For Korea the pricing will be a base of $900. 00 per year, for 4 classes per week, 2 hours per class, we expect class sizes to be 50-150 per class.In America our current pricing is $200. 0 per month, month to month, 2 classes per week, 50 minutes per class. Koreans will look at price per hour over the long term, wanting the most hours per week at the lowest rate with a long term commitment; Americas want to know how cheaply they can Join, and how easily they can quit, more like leasing a car. 83 Promotion: In America martial arts lessons are promoted in a disposable product manner, viewed like a 6 pack of Coke. Coupon driven society demands a call to action for a discount.Teachers are held in higher regards in Korea and it would be a disgrace to offer discounts, promotions, or marketing fluff. The Koreans will want endorsements from martial arts organizations and to see community service done by the staff. When they see us in public, they will approach us, but the Koreans will be completely turned off if the go to corner market and see ââ¬Å"one month of lessons and a f ree uniform for 19. 99â⬠³Ã¢â¬ ¦.. Word of mouth and grassroots promotion only in Korea. BE Place: 52 million people actively practice Tea Swoon Do globally; it is the most popular martial art.Korea in the past has not been accepting of foreign companies opening Dugongs in Korea, but the loss of first place in the last 3 Olympics has changed this. The OIC, International Olympic Committee has removed Baseball, Hockey, and Wrestling as of late. Talk is they will remove Karate, Judo, or Tea Swoon DO. Koreans national sport is Tea Swoon Do, and they are promising more support and resources. They will do whatever it takes to keep this Olympic Sport status. Allowing Americas Best, a proven Junior Olympic Gold Medal winning organization into Korea and supporting its grassroots movement is a given.C Ethical Issues: to us from Korea. The Koreans have warned us that Americans are viewed as fat, lazy, and abrasive. The example proven to us is Korean infant adoption, only for America do the y have a height and weight scale/range for adopting parents from America. 2) Where the money goesâ⬠¦Ã¢â¬ ¦.. Alt has also been brought to our attention from the WTFO that Koreans will need to see a direct line for their tuition to returning to the community. Unlike Americans who will buy a Toyota or Honda and not inquire where the money goes, the Koreans will need to see proof that some of it stays in the community. Sources: None.
Do We Depend Too Much on Computers Essay
As civilization advances we depend more and more upon our invention. There is technology that saved many lives, as well as the one that have destroyed whole cities. Good morning members of the jury, parents, teachers and students. My name is Hugo Mendoza and I will talk about dependency on computers. I choose it because I think people must know all the benefits it brings to our daily life. So, do we depend too much in computers? The answer is that yes, all days we use computers in the business, public services, education, and the most of all entertainment. Even if we donââ¬â¢t note it our life is affected by technology and computers behind all. I will focus about technology in general. After all these years, technology has been evolving constantly; it has facilitated our life in all aspects. First of all, medicine, medicine has improved a lot by the use of technology in hospitals and labs, improving the health of the sick and in bringing up a new medicine for a no before-seen disease quicker than before. There are healing machines that can cure people faster than would take by human hand. Then, there are vehicles such as cars that use the GPS or the monitor to show how much gas is left select music and more. There are even airplanes that can be monitored by computers, and trains that are controlled by computers to prevent a collision. Children play videogames like PS3 or Xbox, with these videogame consoles they will have better experience in computers and will be able to understand better the technology when they grow up. Computers like PCââ¬â¢s and Mac are used by us every day for school, work, and assignments. Students can get into the internet and find any information they want, we can communicate by long distances, and even get an online tutor for any class. By the other hand, technology has been used to create destruction by fabricating guns, war machines and devices like nuclear bombs. This is the other face of technology, and has been used in wars such as World War II. If we continue to make guns, war machine and bombs with the improvement of technology maybe in the future there will be a bomb huge enough to destroy a country or a continent. It would be the end of the world if itââ¬â¢s like that. Scientists fear that in next generations, machines replace completely work done by humans and we doing nothing; they fear that we will not do sport and depend completely on computers. If itââ¬â¢s like that, what would happen if the systems fail to work? There would be complete chaos. Or worse, what would happen if all the systems refuse to listen to peopleââ¬â¢s orders and do what they want? Nobody knows, and we donââ¬â¢t want to know. We have to do something about this. In my opinion, technology and computers have to do a lot in the world we lived today and we canââ¬â¢t live anymore without them, as we got used too much on them. However, technology is a double-edge sword and we should find the way to make this dependency less dangerous and safer for future generations. There most only be new inventions when we really need they are really important, useful and helpful for humans.
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